FIG 1.

Construction and characterization of the E1B 55-kDa- and E4 Orf3-null double-mutant virus AdEasyE1Δ2347-dl341. (A) The origins of the segments of the double mutant virus genome are shown schematically, indicating the positions of the single-pair deletions that prevent production of the E1B 55-kDa or E4 Orf3 protein (ΔE1B and ΔE4 Orf3, respectively) and the EcoRI site exploited in the construction of the mutant. (B) HFFs were infected with 100 PFU/cell AdEasyE1Δ2347 (ΔE1B), dl309, dl341, or the double mutant (ΔE1B/ΔOrf3) for 24 h, and the viral proteins listed on the right were examined by immunoblotting.