FIG 6.

Effect of the E4 Orf3 protein on expression of p53-responsive genes. Total RNA was isolated 30 h after infection of HFFs with 200 PFU/cell AdEasy (WT), AdEasyE1Δ2347 (ΔE1B), or AdEasyE1Δ2347-dl341 (ΔE1B/ΔOrf3) or exposure of uninfected cells to medium containing 125 μM etoposide (E) or from mock-infected cells (M). The concentrations of the p53-responsive genes relative to those of GADPH mRNA were determined by quantitative RT-PCR as described in Materials and Methods. All measurements were made in triplicate, and the means and standard deviations of two independent experiments are shown relative to the values measured in mock-infected cells (A) or in cells infected by the E1B 55-kDa-, E4 Orf3-null double mutant (B).