TABLE 1 .
Summary of pore formation and YopH-TEM translocation data for all shRNAs tested in this study
| Category and gene symbol | Pore formationa |
Stat. sign.b | YopH-TEM translocationa |
Stat. sign. | ||
|---|---|---|---|---|---|---|
| Avg (% vector control) | SEM | Avg (% vector control) | SEM | |||
| ZMAD > 2: FRET+/unsorted, FRET+/FRET− | ||||||
| CALML5 | 114.35 | 7.97 | NS | ND | ||
| CANX | 41.98 | 2.58 | **** | ND | ||
| CCR5 1 | 52.52 | 3.32 | **** | 3.73 | 9.77 | *** |
| CCR5 2 | 80.42 | 4.66 | NS | 41.37 | 13.04 | NS |
| CCR8 | 74.71 | 3.00 | ** | 81.04 | 15.64 | NS |
| CD59 | 56.52 | 3.16 | **** | 18.31 | 13.27 | ** |
| CLTC | 74.44 | 5.06 | * | ND | ||
| COPB1 | 47.38 | 3.66 | ** | ND | ||
| DNAJB6 | 56.17 | 2.42 | **** | 14.65 | 7.97 | ** |
| DNAJC13 | 76.73 | 14.12 | NS | ND | ||
| DNAJC27 | 44.73 | 1.92 | **** | 15.66 | 11.97 | ** |
| DYNC1LI1 | 204.50 | 13.01 | 119.67 | 13.31 | NS | |
| HSP90B1 | 49.85 | 1.93 | *** | ND | ||
| MYO6 | 43.65 | 2.15 | *** | 97.39 | 12.76 | NS |
| PCDHA2 | 31.39 | 2.05 | **** | 62.06 | 4.46 | ** |
| PRKCA | 55.03 | 4.00 | **** | 17.15 | 8.32 | ** |
| RAB27A | 106.91 | 3.84 | NS | 74.62 | 12.49 | NS |
| SEC24B | 81.88 | 4.95 | NS | 38.05 | 16.25 | * |
| SEC63 | 58.11 | 5.44 | ** | ND | ||
| SPTBN5 | 50.43 | 2.93 | **** | 24.89 | 16.79 | ** |
| SUMO3 | 96.86 | 7.82 | NS | 67.33 | 13.87 | NS |
| SYT16 | 38.24 | 4.03 | **** | 58.36 | 3.88 | ** |
| TRAPPC10 | 54.55 | 2.95 | **** | 81.68 | 16.63 | NS |
| ZMAD > 2: FRET/+unsorted | ||||||
| ARFIP2 | 41.19 | 6.69 | *** | 90.86 | 10.20 | NS |
| CAMSAP1 | 49.39 | 3.41 | *** | ND | ||
| DNAJC6 | 62.47 | 12.38 | * | 79.81 | 8.98 | NS |
| HIP1 | 41.87 | 4.35 | **** | ND | ||
| Rock2 | 22.85 | 2.24 | **** | 32.88 | 10.47 | * |
| TTPAL | 85.45 | 18.72 | NS | 80.35 | 4.96 | NS |
| UBQLN3 | 45.55 | 6.58 | *** | 67.91 | 14.74 | * |
| ZMAD < 2: FRET/+unsorted, FRET/+FRET− | ||||||
| APOD | 85.38 | 10.42 | NS | 98.57 | 9.04 | NS |
| COPS8 | 113.80 | 9.58 | NS | 124.32 | 7.88 | NS |
| CYP39A1 | 62.60 | 9.99 | * | 81.44 | 2.39 | NS |
| PRKCA | 127.24 | 18.13 | NS | 95.51 | 3.85 | NS |
| SPTAN1 | 69.24 | 11.74 | NS | 75.92 | 4.11 | NS |
| ARF1 | 38.63 | 5.49 | **** | ND | ||
| ARF6 | 35.14 | 2.77 | **** | ND | ||
| HSPA8 | 49.19 | 6.60 | *** | ND | ||
| RHOA | 25.55 | 2.85 | **** | ND | ||
a
Each shRNA was tested in each assay in triplicate, with experiments repeated at least twice. Data are given as means and standard errors of the means (SEM) of the vector control, pooling all the individual assays for each shRNA.
b
Statistical significance (Stat. sign.) was determined using an ANOVA (P < 0.0001) and a Dunnett’s posthoc test and are indicated as follows: *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; NS, not significant. Some means were not determined (ND).