TABLE 2 .
Expression plasmids
| Plasmid | Source |
|---|---|
| Mammalian expression plasmids | |
| pDest ACAP1-YFP | This study |
| pDest eCFP ADAP1 | This study |
| pDest eCFP ArfGAP1 1–136 | This study |
| pDest ASAP1-YFP 316–665 | This study |
| pDest ASAP2-YFP 1–539 | This study |
| pDest eCFP ARAP3 289–1084 | This study |
| pEGFP Git1 | Addgene 15226 |
| pDest eCFP Arno | Humphreys et al. 2012 (3) |
| pDest eCFP EFA6A 1–645 | Humphreys et al. 2013 (5) |
| pcDNA FLAG ACAP1 | This study |
| pcDNA nHA ACAP1 R448K | This study |
| pcDNA nHA ADAP1 | This study |
| pcDNA nHA ADAP1 R49K | This study |
| pcDNA nHA ArfGAP1 1–136 | This study |
| pcDNA nHA ASAP1 316–665 | This study |
| pcDNA nHA ASAP1 316–665 R497K | This study |
| pcDNA nHA ARAP3 289–1084 | This study |
| pcDNA nHA Arno | This study |
| pcDNA nHA EFA6A 1–645 | Humphreys et al. 2013 (5) |
| pcDNA cHA Arf1 | Humphreys et al. 2012 (3) |
| pcDNA cHA Arf1 Q71L | This study |
| pcDNA cHA Arf1 T31N | This study |
| pcDNA cHA Arf1 T161A | This study |
| pcDNA cHA Arf6 | This study |
| pcDNA cHA Arf6 Q67L | This study |
| pcDNA cHA Arf6 T27N | This study |
| pcDNA cHA Arf6 T157A | This study |
| pmRFP-Rab5 | Addgene 14437 |
| Bacterial expression plasmids | |
| pGEX2T ADAP1 | This study |
| pGEX2T ASAP1 316–665 | This study |
| pbEN-SPB-SET2a ACAP1 268–519 | This study |
| pHis ArfGAP1 1–136 | This study |
| pGEX-GGA3-GAT | Humphreys et al. 2012 (3) |