FIG 2 .

Motility assays in the presence of different doses of BolA protein. (A) To measure motility, bacteria were inoculated in swimming agar plates. The plates were incubated at 37°C for 18 h and photographed. (B) Time course analysis of the bacterial swimming. The dispersion radius was quantified between 12 h and 26 h of incubation at 37°C. One-hundred percent represents the detection limit (whole plate covered by the swimming halo). (C) Observation of E. coli flagella using immunofluorescent staining with antiflagellin antibody. Scale bar represents 2 µm.