TABLE 2 .
Affinity measurement with bio-layer interferometrya
| Protein | Analyte | KD | Ka (1/Ms) | Ka error | Kd (1/s) | Kd error |
|---|---|---|---|---|---|---|
| BolA | fliA | 5.67E−08 | 1.35E04 | 4.91E02 | 7.63E−04 | 5.16E−05 |
| gltA | 5.02E−08 | 3.74E04 | 2.14E03 | 1.88E−03 | 2.36E−04 | |
| mreBCD | 9.34E−08 | 5.69E04 | 2.15E03 | 5.32E−03 | 2.51E−04 | |
| flhDC | ND | ND | ND | ND | ND | |
| Random oligonucleotide | ND | ND | ND | ND | ND | |
| BSA | mreBCD | ND | ND | ND | ND | ND |
a
Equilibrium constants (KD) were determined by bio-layer interferometry using the BLItz system (ForteBio Inc.) according to an advanced kinetics experiment. Ka is the association rate constant, Kd the dissociation rate constant, and KD the equilibrium dissociation constant of the reaction. ND, not determined.