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. 2015 Feb 17;6(1):e02357-14. doi: 10.1128/mBio.02357-14

FIG 1 .

FIG 1 

ISP3-BirA* biotinylates proteins in the parasite IMC. (A) Diagram of the expression cassette encoding ISP3 fused to BirA*, plus a C-terminal 3× HA epitope tag, driven by the ISP3 promoter. (B) IFA of ISP3-BirA*-expressing parasites, grown for 24 h with or without biotin. ISP3-BirA* localizes to the parasite periphery and biotinylates proteins in a biotin-dependent manner. Endogenously biotinylated proteins in the apicoplast are detectable as the background even in the absence of biotin. Bottom panel, ISP3-BirA* biotinylation activity is detectable but not enriched in daughter buds (arrows). Red, mouse anti-HA antibody; green, streptavidin-Alexa 488. Scale bar = 2 µm. (C) Western blot comparing the profile of biotinylated proteins from lysates of parental and ISP3-BirA* parasites.