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. 2015 Feb 17;6(1):e02357-14. doi: 10.1128/mBio.02357-14

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Copyright © 2015 Chen et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 4 — Identification of novel IMC proteins by AC2 BioID. (A) IFA of AC2-BirA*-expressing parasites, grown for 24 h with or without biotin. AC2-BirA* biotinylates proteins in the apical cap in mature (middle) and daughter (bottom, arrows) parasites. Red, mouse anti-HA antibody; green, streptavidin-Alexa488. Scale bar = 2 µm. (B) IFA showing AC2 BioID hits localized to the apical cap demarked by ISP1. Red, rabbit anti-HA antibody; green, mouse anti-ISP1 antibody. (C) Three additional AC2 BioID hits localized to the center and posterior of the IMC, as assessed by costaining with IMC3. Red, mouse anti-HA antibody; green, rat anti-IMC3 antibody.