FIG 2 .

OpaD-expressing gonococci robustly stimulate PMNs to form NETs. NET formation was quantified by taking a ratio of the integrated H2A and DAPI staining area density measurements. NET measurements were plotted against the infection MOI, for PMNs infected with OpaD⁺ P⁺, OpaD⁺ P⁻, Opaless P⁺, and Opaless P⁻ gonococci (A to D, respectively). Data are cumulative from 11 independent experiments using PMNs from one of six different donors. Due to the intrinsic variability of both the primary PMNs and the gonococcus, it is impossible to average these data.