TABLE 2 .
Expression of M. tuberculosis H37Rv key metabolic enzymes within granuloma-like structures from LTBI individuals compared with naive individuals at day 7 postinfectiona
| Gene name/gene product (Rv no.) | Encoded protein function | Metabolic pathway | LTBI versus naive fold difference (mean ± SD) |
|---|---|---|---|
| pckA/phophoenolpyruvate carboxylase (Rv0211) | Gluconeogenic enzyme | Gluconeogenesis | 6.460 ± 5.523 |
| aceA/isocitrate lyase (Rv1915) | Glyoxylate bypass (at the first step) | Glyoxylate shunt cycle | 5.624 ± 1.963** |
| icl/isocitrate lyase (Rv0467) | Glyoxylate bypass (at the first step) | Glyoxylate shunt cycle | 3.07 ± 2.49 |
| fadE5/macyl-CoA dehydrogenase (Rv0244c) | Involved in lipid degradation | Fatty acid beta-oxidation | 4.875 ± 4.066 |
| fadE23/acyl-CoA dehydrogenase (Rv3140) | Involved in lipid degradation | Fatty acid beta-oxidation | 0.400 ± 0.0 |
| fadB/fatty acid oxidation protein (Rv0860) | Involved in fatty acid degradation | Fatty acid beta-oxidation | 3.125 ± 3.0 |
| icd1/isocitrate dehydrogenase (Rv3339c) | Involved in the Krebs cycle | Krebs cycle | 5.333 ± 4.163 |
| sdhC/cytochrome B-556 of succinate dehydrogenase (Rv3316) | Involved in the Krebs cycle | Krebs cycle | 4.873 ± 4.4 |
| ppgk/glucokinase (Rv2702) | Catalyzes the phosphorylation of glucose | Glycolysis | 0.53 ± 0.0 |
| pfkA/phosphofructose kinase (Rv3010c) | Involved in glycolysis | Glycolysis | 0.1 ± 0.0 |
a
Each gene was normalized to the rpoB housekeeping gene, and relative expression levels were determined by the 2−ΔΔCT method. The fold change was determined by comparing expression within granulomas between LTBI individuals and naïve individuals. n = 8 (in triplicate). **, P < 0.01 (Student’s t test). CoA, coenzyme A.