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. 2014 Dec 15;11(4):2578–2584. doi: 10.3892/mmr.2014.3091

Figure 3.

Figure 3

Expression of GPR137 is suppressed by infection with Lv-shGPR137. Analysis of GPR137 messenger RNA expression levels in (A) AGS cells and (B) MGC80-3 cells by reverse transcription-quantitative polymerase chain reaction in the non-infected, Lv-shCon and Lv-shGPR137 groups. GAPDH gene expression was used as the internal control. Analysis of GPR137 protein expression levels in (C) AGS cells and (D) MGC80-3 cells by western blot analysis in the non-infected, Lv-shCon and Lv-shGPR137 groups. GAPDH was used as a loading control. Values are presented as the mean ± standard deviation (n=3). *P<0.05, **P<0.01 vs. Lv-shCon. GPR137, G protein-coupled receptor 137; Con, control; Lv-shCon, control lentivirus; Lv-shGPR137, GPR137 short hairpin RNA-expressing lentivirus.