Table 2.
Primers used for small population and single nuclei qPCR
| Gene | Protein | Locus | Assay type | Primer sequence | Probe | Product length | Efficiency |
|---|---|---|---|---|---|---|---|
| APP, Exon 14 | Amyloid precursor protein | 21q21.3 | SYBR Green | F-TGCACGTGAAAGCAGTTGAAG, R-AAAGATGGCATGAGAGCATCG | N/A | 214 | 0.973 |
| SEMA4A | Semaphorin 4A | 1q22 | SYBR Green | F- ATGCCCAGGGTCAGATACTAT, R-TTCTCCGAGATCCTCTGTTTC | N/A | 177 | 0.997 |
| CCL18 | Chemokine (C–C motif) ligand 18 | 17q11.2 | SYBR Green | F-TTCCTGACTCTCAAGGAAAGG, R-CTGGCACTTACATGACACCTG | N/A | 209 | 1.006 |
| PCDH11X | Protocadherin 11 X-linked | Xq21.3 | SYBR Green | F-TCTTTTGGTCAGTGTTGTGCG, R-CAACAAGTCGCCTATCAGGAC | N/A | 188 | 0.993 |
| APP, Exon 14 | Amyloid precursor protein | 21q21.3 | TaqMan | CGGTCAAAGATGGCATGAGAGCATC*, Assay Hs01255859_cn | FAM-MGB | 91 | 1.040 |
| APP, Exon 3 | Amyloid precursor protein | 21q21.3 | TaqMan | F-GCACTTCTGGTCCCAAGCAT, R-CCAGTTCTGGATGGTCACTG | ROX-IB | 140 | 0.992 |
| SEMA4A | Semaphorin 4A | 1q22 | TaqMan | GTTCAAGGGTATGTGAGGTGAGATG*, Assay Hs00329046_cn_VIC | VIC-MGB | 90 | 1.016 |
*
Denotes probe sequence provided by Life Technologies.