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. 2014 Dec 31;3:e05375. doi: 10.7554/eLife.05375

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© 2014, Dimastrogiovanni et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Author response image 1. — (A) Electrophoretic mobility shift assay (EMSA) with in vitro synthesized, 5' end-labelled RydC and RydC-S1 RNAs (RydC* and RydC-S1*, 4 nM) in the presence of increasing concentrations of Hfq protein as indicated. (B) Preformed Hfq/RNA*(RydC* and RydC-S1*, 4 nM) complexes were incubated with increasing concentrations of cold competitor RNA (RydC-S1 for RydC*; RydC for RydC-S1).