Figure 3. Pharmacological modulation of β-catenin signalling alters distal segment development.

(A) Lgr5-EGFP expression in treated nephrons with segmentation markers. (B) Time-lapse analysis of treated Lgr5^{+/EGFP-IRES-CreERT2} kidneys–arrowheads indicate developing nephrons, red-dashed line indicates ureteric bud (UB). (C) Mean number of Lgr5-EGFP positive nephrons per kidney.

DOI: http://dx.doi.org/10.7554/eLife.04000.005

Figure 3—figure supplement 1. Lgr5 expression domain in heterozygous and homozygous Lgr5^{+/EGFP-IRES-CreERT2} kidneys.

Cultured Lgr5^{+/EGFP-IRES-CreERT2} kidneys stained for segmentation marker Jag1 and epithelial marker Cdh1. (A and C) Lgr5^{+/EGFP-IRES-CreERT2} heterozygous kidneys and kidneys and (B and D) Lgr5 ^{EGFP-IRES-CreERT2/EGFP-IRES-CreERT2} homozygous kidneys and nephrons display normal segmentation. Homozygous tissue displays stronger EGFP expression as expected but nephrons appear morphologically normal.

DOI: http://dx.doi.org/10.7554/eLife.04000.006