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Kidneys cultured in control medium, 4 µM LDN-193189, 20 µM Ly294003, 1.5 µM CHIR, 4 µM LDN-193189, and 1.5 µM CHIR, or 20 µM Ly294003 and 1.5 µM CHIR. Timing and scales are as specified.
