Author response image 2.

qRT-PCR performed on FAC-sorted cells obtained after electroporation at E15.5 with an Intron-RED control plasmid (black bars) or the miR-128-2 expression plasmid pre-miR-128-1-RED (gray bars). dsRed positive cells were collected, RNA isolated and qRT-PCR performed using Oaz1 as reference mRNA. PHf6, Szrd1 were readily detected, quantification is shown. Szrd1 as a known target of miR-128 is the positive control, β-actin the negative control. Although a clear trend is observed, the reduction of PHf6 mRNA in response to ectopic miR-128 is not statistically significant (unpaired t-test).