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. 2014 Dec 31;3:e03751. doi: 10.7554/eLife.03751

Author response image 3.

Author response image 3.

EBC1 cells were treated with the pan‐class‐I PI3K inhibitor, and/or the AKT inhibitor GSK690693. Cells were allowed to grow for 96 hours and assessed for cell death following this incubation period by the trypan blue method (left). A duplicate set of samples was analysed by western blot to document drug activity. Note that GSK690693 does not protect (red arrow) from PI3K‐inhibitor‐induced cell death (green arrow).