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Chloride channel blockers decrease the rate of cisplatin-induced apoptosis in MG-63 human osteosarcoma cells. The cells were treated with cisplatin (CDDP; 2 μg/ml) in Dulbecco’s modified Eagle’s medium alone, or in combination with chloride channel blockers 5-nitro-2-(3-phenylpropylamino)-benzoate (NPPB; 100 μmol/l) or tamoxifen (20 μmol/l) for 72 h. The data represent the mean percentage of inhibition of apoptosis ± standard error of six experiments. **P<0.01, vs. the control.
