Figure 2.

Chloride channel blockers increase proliferation of cisplatin-treated MG-63 human osteosarcoma cells. The cells were cultured in 96-well culture plates (at a density of 2,500 cells/well) in control medium overnight (~16–18 h), and were then incubated in control medium (Ctrl) or medium containing cisplatin (CDDP; 2 μg/ml) alone, or in combination with 5-nitro-2-(3-phenylpropylamino)-benzoate (NPPB; 100 μM) or tamoxifen (20 μM) for 36 or 72 h. The relative cell numbers were detected by an MTT assay and the optical density (OD) of the cells was measured. The figure shows the comparison of cell growth to standardized OD values at different time points in MG-63 cells. The data represent the mean ± standard error of three experiments. ^**P<0.01, vs. the control.