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. 2015 Feb 10;4:e05178. doi: 10.7554/eLife.05178

Figure 4. Spatio-temporal association of SPREADs with cell divisions.

(A and B) Left panels show frequencies of SPREADs (blue) and cell divisions (red) during periods with frequent SPREADs (A) and less frequent SPREADs (B). Right panels show mapping of the centres of SPREADs (blue circle) and cell divisions (red circle) observed during each imaging period. Shown are single frames of CFP images of a 0.213 mm2 viewfield. Individual mouse identification numbers, sex and age are shown on the top of the left panels. Scale bar, 100 μm. (C) The frequency of cell division in and out of the SPREAD area. SPREAD area was defined as the area within 100 μm from the origin and within 1 hr of the onset of the SPREAD. *p < 0.05 (paired Student's t test). (D) Time-lapse images of the yellow square region in (A), showing three SPREADs emerging from the same spot.

DOI: http://dx.doi.org/10.7554/eLife.05178.012

Figure 4.

Figure 4—figure supplement 1. Frequencies of SPREAD and cell division in the steady-state ear skin of eleven mice.

Figure 4—figure supplement 1.

(A) Ear skin of eleven Eisuke mice were subjected to imaging as described in Figure 1. Time-lapse imaging was aborted when the body temperature and breathing conditions of mice deteriorated. Frequencies of SPREADs (bars) and cell divisions (lines) during imaging are shown with the individual mouse identification numbers, sex and age on the top. (B) Basal ERK activity in mice with frequent SPREAD (mice #102, 107, 112, 115, and 117) and infrequent SPREAD (mice #113, 114, and 120).
DOI: http://dx.doi.org/10.7554/eLife.05178.013
Figure 4—figure supplement 2. Steady-state backskin with frequent SPREADs and cell divisions.

Figure 4—figure supplement 2.

Left panel shows the frequencies of SPREADs (bars) and cell divisions (lines) during imaging. Mouse identification number, sex and age are shown on the top. Right panel shows mapping of SPREADs (blue circles) and cell divisions (red circles) observed during 14-hr imaging period in the viewfield of 0.213 mm2. Scale bar, 100 μm.
DOI: http://dx.doi.org/10.7554/eLife.05178.014
Figure 4—figure supplement 3. Monte Carlo simulation of SPREAD distribution.

Figure 4—figure supplement 3.

Left panel, the distribution of SPREADs in the viewfield of mouse #102. Right panel, the histogram of average nearest neighbor distance between each SPREAD centre and its nearest neighbor's centre obtained by 100,000 trials of Monte Carlo simulations of SPREAD distributions.
DOI: http://dx.doi.org/10.7554/eLife.05178.015