Table 2.
Summary of single-molecule RNA FISH statistics
DOI: http://dx.doi.org/10.7554/eLife.05007.012
Table 2—source data 1. Single-molecule RNA FISH values from three independent experiments.
elife05007s002.xlsx (412.9KB, xlsx)
DOI: 10.7554/eLife.05007.013
| RNA | Genotype | Mean spots per cell | Variance | % Cells with >0 spots | % Cells with >1 spot |
|---|---|---|---|---|---|
| cre | HMLα::cre, sir4Δ | 12 ± 2 | 53 ± 9 | 98 ± 1 | 95 ± 2 |
| HMLα::cre, sir1Δ | 5 ± 1 | 58 ± 13 | 46 ± 7 | 41 ± 7 | |
| HMLα::cre, SIR+ | <0.005 | – | 0.42 ± 0.07 | 0.04 ± 0.01 | |
| No cre, SIR+ | <0.003 | – | 0.3 ± 0.1 | 0 | |
| KAP104 | HMLα::cre, sir4Δ | 7.3 ± 0.4 | 11 ± 1 | 99.0 ± 0.3 | 97.2 ± 0.8 |
| HMLα::cre, sir1Δ | 7.1 ± 0.2 | 10.8 ± 0.9 | 99.28 ± 0.04 | 97.5 ± 0.1 | |
| HMLα::cre, SIR+ | 6.1 ± 0.2 | 7.8 ± 0.3 | 99.1 ± 0.2 | 96.5 ± 0.8 | |
| No cre, SIR+ | 5.9 ± 0.5 | 7.1 ± 0.5 | 99.0 ± 0.7 | 96 ± 2 |
All values represent the mean of three independent experiments ± standard error of the mean. Full dataset is shown in Table 2—source data 1.