Figure 1. Inter- and intra-animal variability of ultradian activity rhythms across time in circadian incompetent mice.
(A) Representative double-plotted actograms of running wheel activity in SCN-lesioned (SCNx) and Bmal1−/− mice in DD. (B) Dot plot of locomotor period length in DD based on Lomb-Scargle periodogram analysis of seven consecutive days of activity recording (N = 65 for Bmal1−/− and N = 48 for SCNx; t111 = 0.2785, p = 0.78, unpaired t-test). (C) Intra-animal period variability expressed as mean ± SD for each animal, ranked according to mean period length derived from continuous wavelet transforms (CWT) for the 1–12-hr frequency range (same animals and timespans for calculation as in B). (D) CWT-heatmaps showing decibel scaled and normalized amplitude of oscillations according to frequency and time with black traces indicating the ridge of local amplitude maxima.