Figure 4. H-NS effects on transcript elongation were reduced at ≥30°C.
Densitometry profiles of transcripts produced at 25°C, 30°C, or 37°C, 8 mM Mg2+ and 30 μM each NTP from the λPR-bgl template in the presence of 66 H-NS/kb (bridged filaments). Samples were removed at 2, 4, 8, 16, and 32 min after addition of NTPs and separated by denaturing PAGE. Insets, mean transcript lengths at various time points plotted were averaged from two independent experiments.
Figure 4—figure supplement 1. Electrophoretic gel image showing reduced H-NS effects on transcription elongation at ≥30°C.
10 nM-halted ECs formed on the λPR-bgl template were incubated with 66 H-NS/kb in 8 mM Mg2+ for 20 min at 25°C, 30°C, or 37°C. 30 μM NTPs were added, time points were taken at 2, 3, 4, 8, 16, and 32 min and resolved by PAGE. M denotes labeled MspI-digested pBR322 marker. RO indicates template run-off.