Fig 2.

Neutrophil and monocyte CD11b in response to lipopolysaccharide (LPS) and activated protein C (APC) modulation in neonatal encephalopathy (NE): whole blood from healthy adult controls (n = 15), cord controls (n = 8) and NE patients (n = 23) incubated with LPS ± APC for 1 h. Neutrophils were assessed for CD11b using a phycoerythrin (PE)-labelled monoclonal antibody (mAb) and mean channel fluorescence analysed using flow cytometry. The neutrophil and monocyte populations were selected based on their scatter profile (forward- and sideways-scatter): (a) neutrophil CD11b, *P < 0·05 versus control; (b) monocyte CD11b, *P < 0·05 versus adult and cord controls; **P < 0·05 versus controls.