Fig 3.

Neutrophil and monocyte Toll-like receptor (TLR)-4 in response to lipopolysaccharide (LPS) and activated protein C (APC) modulation in neonatal encephalopathy (NE): whole blood from healthy adult controls (n = 15), cord controls (n = 8) and NE patients (n = 23) was incubated with LPS ± APC for 1 h. Cells were then labelled with Alexa Fluor 647 TLR-4 monoclonal antibody (mAb) and mean channel fluorescence analysed using flow cytometry. The neutrophil and monoclonal populations were selected via flow cytometry based on their scatter profile. (a) Neutrophil TLR-4: *P < 0·05 versus control; **P < 0·05 versus LPS response. (b) Monocyte TLR-4: no significant differences.