Fig 2.

Depletion of regulatory T cells (T_regs) with a rat anti-mouse CD25 monoclonal antibody. (a) Mice were treated with rat anti-mouse CD25 monoclonal antibody (mAb) on days −16 and −13 prior to challenge. Depletion was confirmed using intracellular forkhead box protein 3 (FoxP3)-staining followed by flow cytometric analysis on days −12, −5 and 10. Mice were subsequently sensitized on day −5, challenged on day 0 and the ear-swelling response was measured 10 days post-challenge. (b) %CD25⁺FoxP3⁺ cells of CD4⁺ cells in blood checked by flow cytometry at days −12, −5 and 10 in relation to challenge in groups treated with 100, 200 and 400 μg anti-CD25 mAb/mouse, respectively, as well as in a untreated control group. (c) Ear-swelling response in groups treated with 0 (untreated control), 100, 200 and 400 μg anti-CD25 mAb/mouse in 10 days post-challenge. (d,e) %CD4⁺ cells of CD45⁺ cells in the blood at days −12 (d) and −5 (e) in groups treated with 100, 200 and 400 μg anti-CD25 mAb/mouse, respectively, as well as in an untreated control group. Data are depicted as mean ± standard error of the mean, n = 5/group.