Fig 5.
Depletion of regulatory T cells (Tregs) augments the level of local and systemic inflammatory mediators early in the response. (a–h) Homogenates of inflamed ear tissue were analysed for their content of a range of selected cytokines and chemokines at days 3 and 8 after challenge both in Treg-depleted mice and in non-depleted control mice. (a) Tumour necrosis factor (TNF)-α; (b) interleukin (IL)-6; (c) IL-1β; (d) interferon (IFN)-γ; (e) IL-4; (f) IL-10; (g) macrophage inflammatory protein (MIP)-2; (h) IFN-γ-induced protein 10 (IP-10); (i,j) serum from Treg-depleted mice and non-depleted control mice was analysed for acute-phase reactants serum amyloid P (SAP) (i) and haptoglobin (j) at days 3 and 8 after challenge. Data are depicted as mean ± standard error of the mean. *P < 0·05, **P < 0·01 and ***P < 0·001, n = 5/group.