Fig 4.

Effect of complement receptor 2 (CR2)-fH on development of fibrosis and local complement activation in mice with chronic dextran sulphate sodium (DSS)-induced colitis. (a) Picrosirius red staining of colon sections isolated from mice at end of fourth cycle rest period was used to quantify percent collagen as a marker of fibrosis development. Data expressed as percentage of collagen per five high-powered fields (HPF). Mean ± standard error of the mean (s.e.m.); n = 7–10, *P < 0·05; **P < 0·01. (b,c) C5a levels were assessed in colon homogenates at the end of fourth cycle of DSS treatment (b) or at end of fourth cycle rest period (c). Mean ± s.e.m.; a, n = 5–6; b, n = 4 (representative of two independent experiments). *P < 0·05.