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. 2015 Feb 16;179(3):509–519. doi: 10.1111/cei.12469

Fig 3.

Fig 3

Cytokine-activated renal tubular epithelial cells (TECs) inhibit CD4+ and CD8+ T cell proliferation in a cell–cell contact-dependent manner irrespective of TEC-derived-IDO activity. Interferon (IFN)-γ/tumour necrosis factor (TNF)-α tubular epithelial cells (TEC) were co-cultured with anti-CD3/CD28-activated peripheral blood mononuclear cells (PBMC) (TEC : PBMC ratio of 1:2·5, n = 6). (a) CD4+ T cell proliferation was inhibited significantly by activated TECs. 1-methyl-L-tryptophan (1-L-MT)recovered CD4+ T cell proliferation only partially (*P < 0·05). (b) Proliferating CD8+ T cells were inhibited significantly by activated TECs. 1-L-MT did not recover CD8+ T cell proliferation at all. (c) IFN-γ/TNF-α TECs were cultured with anti-CD3/CD28-activated PBMC using Transwell membranes (TEC : PBMC ratio of 1:2·5, n = 6). Simultaneous Transwell cultures in the absence or presence of 1-L-MT were performed. Activated TECs did not affect CD4+ T cell proliferation and CD8+ T cell proliferation in Transwell experiments. 1-L-MT did not affect CD4+ and CD8+ T cell proliferation.