Skip to main content
. 2015 Jan 13;172(5):1222–1236. doi: 10.1111/bph.12998

Figure 1.

Figure 1

MLN4924 sensitizes monocytes and iDCs for TNF-induced cell death. (A) Monocytes, iDCs and mDCs were challenged overnight with 5 μM MLN4924, and total lysates were analysed by Western blotting for the presence of the indicated proteins. (B) Cells were stimulated in triplicate with MLN4924 (5 μM), and next day cellular viability was determined using the MTT assay. (C) Cells were stimulated in triplicate in 96-well plates with the indicated concentrations of TNF in the presence and absence of 5 μM MLN4924. MLN4924 was added 30 min before stimulation with TNF. Next day, cellular viability was again quantified with MTT and normalized against samples treated with a mixture of cytotoxic reagents.