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. 2015 Jan 13;172(5):1222–1236. doi: 10.1111/bph.12998

Figure 2.

Figure 2

MLN4924/TNF-induced cell death is mediated by TNFR1. (A) Human monocytes, iDCs and CD40L-matured DCs (mDCs) were analysed by FACS evaluation for the cell surface expression of the indicated proteins. (B) iDCs were stimulated in triplicate in 96-well plates with the indicated concentrations of the TNFR1-specific TNF mutant TNF(32W/86T) or of the selectively TNFR2-activating TNF variant TNC-scTNF(143N/145R) in the presence and absence of 5 μM MLN4924. Next day, cellular viability was quantified and normalized against samples treated with a mixture of cytotoxic reagents. (C) Untreated and MLN4924-treated iDCs were challenged with the indicated concentrations of Fc-CD95L and assayed the next day for viability using the MTT assay.