Figure 3.

MLN4924 sensitizes monocytes and iDCs for TNF-induced apoptosis and necroptosis. (A) Monocytes and iDCs were challenged with TNF (100 ng·mL⁻¹) and MLN4924 (5 μM) in the presence of the indicated mixtures of nec-1 (90 μM), NSA (2.5 μM) and zVAD-fmk (50 μM). Next day, cells were assayed for cellular viability using the MTT assay. (B and C) Monocytes and iDCs were treated with TNF (200 ng·mL⁻¹) and MLN4924 (5 μM) in the presence of the indicated mixtures of nec-1 (90 μM) and zVAD-fmk (50 μM) for 8 h, and total cell lysates were subjected to Western blot analysis for processing of the indicated proteins.