Figure 4.

In the presence of zVAD-fmk, MLN4924 sensitizes monocytes for endogenous TNF-induced necroptosis. (A) Monocytes and iDCs were analysed by FACS for their cell surface expression of TNF. (B) Monocytes and iDCs were challenged with the indicated concentrations of MLN4924 in the presence and absence of zVAD-fmk (50 μM). The next day, cellular viability was assayed by MTT assay. (C) Monocytes were seeded on 96-well plates in the presence and absence of zVAD-fmk (50 μM), Humira (10 μg·mL⁻¹), Enbrel (10 μg·mL⁻¹), nec-1 (90 μM) or NSA (2.5 μM). Thirty minutes after adding the various reagents, cells were treated with MLN4924 (5 μM) and the next day cellular viability was assayed using the MTT assay.