Figure 5.

MLN4924 inhibits TNF-induced maturation of DCs independently of cell death induction. (A and B) iDCs were seeded in medium supplemented with nec-1 (90 μM) and zVAD-fmk (50 μM). Cells were then stimulated with TNF (500 ng·mL⁻¹) and MLN4924 (5 μM) and were investigated after 48 h for DC maturation by analysing cell surface expression of CD83 and CD86 by FACS (A) and after 24 h for the secretion of IL-12 and IL-6 by elisa (B). (C) iDCs were stimulated with 500 ng·mL⁻¹ TNF for the indicated time in the presence and absence of MLN4924 (5 μM). Total cell lysates were finally analysed by Western blotting for phosphorylation and degradation of IκBα.