Figure 4.

Effects of RS9 on cellular damage induced by LPS in RAW 264.7 cells. (A) Effects of bardoxolone methyl and RS9 on LPS-induced increase in hydrogen peroxide in RAW 264.7 cells. Addition of 10 ng·mL⁻¹ LPS increased the production of hydrogen peroxide, and treatment with the compounds for 48 h suppressed the increase. Triplicate results, **P < 0.01 versus no LPS, †P < 0.05 versus LPS without compounds, Tukey's test. (B) Effects of compounds on cellular viability in LPS-treated RAW 264.7 cells. Experimental conditions were the same as in Figure 3G. Triplicate results, *P < 0.05, **P < 0.01 versus LPS only, Dunnett's test. (C) Translocation of Nrf2 protein to the nucleus in RAW 264.7 cells. An increase in translocated Nrf2 was observed in both groups. **P < 0.01 versus no compounds, Dunnett's test.