Figure 7.

SGE-201 presence reveals differences between memantine and ketamine neuroprotection during hypoxia. A. Hippocampal cultures were exposed to hypoxia (95% N₂, 5% CO₂, see Methods), for 2.5 h. Cell death was assessed 24 h post insult using PI (3 μM). Images depict representative fields (10×) of control, hypoxia, and memantine and ketamine dishes; red dots indicate dead (i.e. PI positive) neurons. For analysis, Hoescht stain (5 μM) was also included to identify the total neuronal population (not shown). B. Without SGE-201, memantine and ketamine protected similarly against the hypoxic insult. P = 0.035, paired t-test, n = 12. C. In the presence of 0.2–1.0 μM SGE-201, a significantly stronger difference emerged between memantine and ketamine neuroprotection. *P < 0.05, within experiment paired t-test; P < 0.001 for a significant interaction between SGE-201 and blocker neuroprotection: two-way anova, n = 8.