Table I.
Band assignments of major absorptions in the Fourier transform infrared spectra of the SW620 cells in the 4,000-900 cm−1 region.
| Peak number | Frequency (cm−1) | Assignments |
|---|---|---|
| 1 | 3,290 | νO-H, νN-H (water, protein) |
| 2 | 2,925 | C-H stretching bands νas CH2 (lipid) |
| 3 | 2,852 | νs CH2 of lipids |
| 4 | 1,740 | C=O stretching bands (lipids) |
| 5 | 1,640 | Amide I (of proteins in α-helix conformation) and water |
| 6 | 1,540–1,580 | Amide II (an N-H bending vibration coupled to C-N stretching) |
| 7 | 1,454 | CH3 bending vibration (lipids and proteins) |
| 8 | 1,410 | δC-H, δC-O-H, amino acid residues |
| 9 | 1,310–1,320 | Amide III band components of proteins |
| 10 | 1,240 | Asymmetric PO2− stretching in RNA |
| 11 | 1,121 | C-O(H) stretching bands, threonine and tyrosine |
| 12 | 1,080–1,085 | C-O-C stretching (nucleic acids and phospholipids) indicates a degree of oxidative damage to DNA |
| 13 | 1,040 | C-O stretching bands, nucleic acid molecule polysaccharide |