Figure 2. The noncanonical binding of BiP ATPase domain to Ire1 and Perk is independent of nucleotides.
(A–B) MST analysis showing sigmoidal binding curves for full-length BiP interaction with (A) Ire1 luminal domain and (B) Perk luminal domain in the presence of 10 mM ATP, ADP, AMPPNP and in the absence of nucleotides. (C) List of Kd values (μM ± SE) for Ire1 and Perk luminal domain interactions with full-length BiP in the presence of nucleotides for the binding curves represented in A and B. Binding between luminal domains and BiP was not affected by the presence of the various nucleotides.