Figure 4. MK2 and MK3 mediate phosphorylation of Beclin 1 S90.
(A) In vitro kinase activity of MK3 using indicated Beclin 1 peptides as a substrate. (B) In vitro kinase activity of MK2, MK3, and MK5 using the wild-type (WT) Beclin 1 peptide shown in A as a substrate. (C) In vitro kinase activity of MK2 and MK3 using Flag epitope-tagged wild-type Beclin 1 or Beclin 1 S90 purified from HEK293T cells as a substrate. See also Figure 4—figure supplement 1 and Supplementary file 1.
Figure 4—figure supplement 1. Results of in vitro kinase screen using the Beclin 1 83–97 peptide as a substrate.
Kinases included in screen are listed in alphabetical order on the x-axis. See Supplementary file 1 for details.