Table 1.

Mutant strains used for the whole cell membrane rigidity measurements

DOI: http://dx.doi.org/10.7554/eLife.05663.004

Gene	Function	Deletion effect
shc (hpnF)	Cyclization of squalene to form C30 hopanoids (diploptene and diplopterol)	No hopanoid production and accumulation of squalene
hpnH	Addition of adenosine to diploptene to generate adenosylhopane, a precursor for extended hopanoid production	No extended hopanoid production, accumulation of C30 hopanoids
hpnG	Removal of adenine from adenosyl hopane	No BHT and aminoBHT production, accumulation of adenosylhopane
hpnN	An IM transporter that transports hopanoids to the outer membrane	Absence of hopanoids in the OM and accumulation of hopanoids in the IM
hpnO	Production of aminoBHT	No aminoBHT production
hpnP	Methyl transfer to A ring at C-2	No hopanoid methylation

The function of the gene and the effect of its deletion are listed.