Table 3.
Annotation of phospholipids identified by LC-MS analyses (see Figure 7)
| Compound | RT (min) | [M+H]+ | [M−C3H7O2HPO4]+ | [M+NH4]+ |
|---|---|---|---|---|
| PG32:1 | 4.39 | 549.4895 | ||
| PG34:2 | 4.89 | 575.505 | ||
| PC34:2 | 5.94 | 758.5694 | ||
| PG34:1 | 6.11 | 577.5208 | ||
| PG36:2 | 6.2 | 603.5364 | ||
| PE34:2 | 6.38 | 716.5225 | ||
| PC_cyc35:1 | 6.76 | 772.5851 | ||
| PG_cyc35 | 6.95 | 591.5364 | ||
| PG_cyc37:1 | 7.05 | 617.5521 | ||
| PC(35:2) | 7.06 | 772.5851 | ||
| PE_cyc35:1 | 7.28 | 730.5381 | ||
| PC34:1 | 7.51 | 760.5851 | ||
| PC36:2 | 7.65 | 786.6007 | ||
| PG(17:0/17:0) | 7.86 | 579.5364 | ||
| PG36:1 | 7.99 | 605.5521 | ||
| PE34:1 | 8.14 | 718.5381 | ||
| PE36:2 | 8.27 | 744.5538 | ||
| PC_cyc35 | 8.58 | 774.6007 | ||
| PC_cyc37:1 | 8.72 | 800.6164 | ||
| PE_cyc35 | 9.28 | 732.5538 | ||
| PE_cyc37:1 | 9.42 | 758.5694 | ||
| PC(17:0/17:0) | 9.75 | 762.6007 | ||
| PC36:1 | 9.91 | 788.6164 | ||
| PE(17:0/17:0) | 10.53 | 720.5538 | ||
| PE36:1 | 10.71 | 746.5694 | ||
| PC_cyc37 | 11.23 | 802.632 | ||
| PE_cyc37 | 12.1 | 760.5851 | ||
| PC36:0 | 12.79 | 790.634 | ||
| PE36 | 12.98 | 748.5851 | ||
| PC36:4 | 13.39 | 782.569 | ||
| CL70:4 | 15.09 | 1447.0373 | ||
| CL68:3 | 15.1 | 1421.0217 | ||
| CL72:4 | 15.31 | 1475.0686 | ||
| CL70:3 | 15.34 | 1449.053 | ||
| CL68:2 | 15.35 | 1423.0373 | ||
| CL72:3 | 15.58 | 1477.0843 | ||
| CL70:2 | 15.6 | 1451.0686 |
The types of lipids (PC: phosphatidylcholine, PE: phosphotidylethanolamine, PG: phosphatidylglycerol, CL: cardiolipin, cyc: cyclopropanation; the first number indicates the total number of carbon of the fatty acid chains and the second number indicates the number of double bonds in these chains) and their retention time (RT, min) and m/z value of the base peak are shown. For PC and PE, the base peak is the proton adduct and for CL, the base peak is the ammonium adduct. For PG, the base peak indicates a loss of glycerophosphate (−171 m/z).