Table 4.
Phospholipid compositions in the inner membrane (IM) and outer membrane (OM) of R. palustris TIE-1 WT and ΔhpnP analyzed by LC-MS
Weight % of TLE | |||||
---|---|---|---|---|---|
Compound | RT (min) | WT IM | WT OM | ΔhpnP IM | ΔhpnP OM |
PC36:2 | 7.65 | 5.15 ± 0.47 | 5.34 ± 0.26 | 5.44 ± 0.57 | 5.94 ± 0.48 |
PC_cyc37:1 | 8.72 | 4.33 ± 0.45 | 4.01 ± 0.48 | 4.07 ± 1.13 | 3.96 ± 1.24 |
PC36:1 | 9.91 | 2.90 ± 0.3 | 1.84 ± 0.52 | 2.59 ± 0.93 | 2.15 ± 1.08 |
PC34:1 | 7.51 | 2.66 ± 0.22 | 2.23 ± 0.33 | 2.53 ± 0.58 | 2.38 ± 0.57 |
PC34:2 | 5.94 | 1.56 ± 0.12 | 1.24 ± 0.44 | 1.41 ± 0.51 | 1.44 ± 0.48 |
PC_cyc35 | 8.58 | 0.23 ± 0.01 | 0.14 ± 0.05 | 0.20 ± 0.07 | 0.17 ± 0.1 |
PC_cyc35:1 | 6.76 | 0.12 ± 0.01 | 0.07 ± 0.02 | 0.11 ± 0.04 | 0.09 ± 0.06 |
PC(35:2) | 7.06 | 0.09 ± 0.01 | 0.05 ± 0.03 | 0.08 ± 0.03 | 0.06 ± 0.05 |
PC_cyc37 | 11.23 | 0.07 ± 0.01 | 0.04 ± 0.01 | 0.06 ± 0.02 | 0.05 ± 0.03 |
PC36:0 | 12.79 | 0.02 ± 0 | 0.01 ± 0 | 0.02 ± 0.01 | 0.01 ± 0.01 |
Sum | 17.12 | 14.96 | 16.50 | 16.25 | |
PE_cyc37:1 | 9.42 | 4.92 ± 0.36 | 3.36 ± 0.94 | 4.68 ± 1.5 | 4.18 ± 1.64 |
PE34:1 | 8.14 | 2.49 ± 0.19 | 1.44 ± 0.49 | 2.28 ± 0.84 | 1.82 ± 0.92 |
PE36:1 | 10.71 | 2.29 ± 0.28 | 1.08 ± 0.38 | 2.05 ± 0.85 | 1.43 ± 0.95 |
PE36:2 | 8.27 | 1.88 ± 0.21 | 1.22 ± 0.46 | 1.74 ± 0.65 | 1.59 ± 0.72 |
PE34:2 | 6.38 | 0.43 ± 0.02 | 0.23 ± 0.08 | 0.42 ± 0.16 | 0.33 ± 0.21 |
PE_cyc35 | 9.28 | 0.20 ± 0.02 | 0.11 ± 0.04 | 0.19 ± 0.07 | 0.15 ± 0.09 |
PE_cyc35:1 | 7.28 | 0.12 ± 0.01 | 0.06 ± 0.02 | 0.11 ± 0.05 | 0.09 ± 0.06 |
PE_cyc37 | 12.1 | 0.07 ± 0.01 | 0.04 ± 0.01 | 0.07 ± 0.02 | 0.05 ± 0.03 |
PE36 | 12.98 | 0.04 ± 0 | 0.03 ± 0.01 | 0.04 ± 0.01 | 0.03 ± 0.01 |
Sum | 12.44 | 7.58 | 11.58 | 9.67 | |
PG36:2 | 6.2 | 2.59 ± 0.24 | 1.68 ± 0.54 | 2.25 ± 0.67 | 1.87 ± 0.8 |
PG36:1 | 7.99 | 1.13 ± 0.08 | 0.69 ± 0.19 | 0.98 ± 0.31 | 0.81 ± 0.35 |
PG34:1 | 6.11 | 0.77 ± 0.17 | 0.5 ± 0.2 | 0.6 ± 0.24 | 0.51 ± 0.19 |
PG_cyc37:1 | 7.05 | 0.11 ± 0.02 | 0.07 ± 0.05 | 0.08 ± 0.02 | 0.07 ± 0.04 |
PG34:2 | 4.89 | 0.05 ± 0 | 0.03 ± 0.01 | 0.04 ± 0.01 | 0.03 ± 0.02 |
PG_cyc35 | 6.95 | 0.02 ± 0 | 0.01 ± 0 | 0.02 ± 0.01 | 0.01 ± 0.01 |
PG32:1 | 4.39 | 0.00 ± 0 | 0.01 ± 0 | 0 ± 0 | 0.01 ± 0 |
Sum | 4.67 | 3 | 3.97 | 3.31 | |
PC + PE + PG | Total % of TLE | 34.23 | 25.54 | 32.06 | 29.23 |