Figure 9. AMOT p130 is required for an early stage of HIV-1 assembly/budding.
(A) Transmission electron microscopic (TEM) images of HIV virions budding from 293T cells depleted of TSG101. Panel 1: wide view. Panel 2: expanded view. Scale bars here and in part B are 100 nm. (B) TEM images of HIV virions budding from 293T cells depleted of AMOT p130 and p80. Panel 1: wide view. Panel 2: expanded view. (C) Quantification of the release and maturation status of HIV-1 virions associated with cells that were treated with a control siRNA (black bars), depleted of TSG101 (red), depleted of AMOT (teal), or depleted of AMOT and re-expressing AMOT p130 from an siRNA-resistant construct (light blue). This experiment was repeated twice with similar results, and error bars were derived by quantifying three separate sets of >100 virions each from one of the experiments. (D) Quantification of the completeness of virions budding from cells treated with a control siRNA (black triangles), depleted of TSG101 (red squares), or depleted of AMOT (teal diamonds). The extent of the Gag shell arc (in degrees) was measured from TEM images of 500 budding virions of each type, the measurements were binned into the intervals shown below the x-axis, and the virion numbers in each bin are shown in the plot.