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. 2014 Dec 11;11(4):2477–2485. doi: 10.3892/mmr.2014.3084

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Double immunofluorescence staining for (A) ID1 (green), (B) GAD67 (red), (C) ID1+GAD67 (merged image), (D) ID4 (green), (E) Iba-1 (red) and (F) ID4+Iba-1 (merged image) in the Cornu Ammonis region CA1 5 days after ischemia-reperfusion. ID1⁺ cells colocalize with GAD67⁺ GABAergic interneurons (C, arrows); ID4⁺ cells colocalize with GFAP⁺ astrocytes (F, arrows). ID, inhibitors of DNA binding/differentiation proteins; GAD67, glutamic acid decarboxylase 67; GABA, γ-aminobutyric-acid; Iba-1, ionized calcium-binding adapter molecule 1; GFAP, glial fibrillary acidic protein; SO, stratum oriens; SP, stratum pyramidale; and SR, stratum radiatum. Scale bar=20 μm.