Figure 8.

OCT4 gives hHFMSCs a potential for transdifferentiating into erythroid cells by triggering pluripotent state and reexpressing erythroid development genes. (a) OCT4 plays a critical role in transdifferentiation of hHFMSCs into erythroid cells. (b) RT-qPCR detection of OCT4 target genes in transdifferentiation of hHFMSC into erythroid cells. Exogenous OCT4 genes were transduced into hHFMSCs in manner of MOI: 160 (named hHFMSC^OCT4), and hHFMSCs^OCT4-d21 were derived from hHFMSC^OCT4 by hematopoietic cytokines culture for 21 days. (A) Expression of OCT4 target genes associated with pluripotency. (B) Expression of OCT4 target genes associated with erythroid development. ^* P < 0.05; ^** P < 0.01; n.d., undetected.