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. Author manuscript; available in PMC: 2015 Feb 23.

Published in final edited form as: Biochim Biophys Acta. 2010 May 25;1800(9):906–911. doi: 10.1016/j.bbagen.2010.05.002

Fig. 2 — K-ATPase activity in the colonic membrane vesicles of mice. (A) Colonic K-ATPase activity was determined in the colonic membrane vesicles of HKα₂ wild type (WT), HKα₂ knockout (α₂ KO), and HKβ knockout (β KO) mice. Values (in micromole ATP hydrolyzed per mg membrane protein per hour) are means ± se from the indicated number of animals (n). Comparisons between groups were performed by Student’s t-test. NS stands for no statistically significant difference between groups. (B) K-dependent ATPase activity was measured in the colonic membrane vesicles extracted from wild type mice. Maximal stimulation of K-ATPase activity was reached at K⁺ concentration of 200 μM. Inset, Lineweaver-Burk plot of 1/V versus 1/[S] indicates apparent K_m of 40 μM for the colonic K-ATPase activity. (C) Colonic K-ATPase activity was measured in the absence of inhibitor or in the presence of 2 mM ouabain, or 100 μM Sch28080, or 100 μM orthovanadate. Values are means ± se from the indicated number of animals (n). * indicates P<0.001, statistically different from the colonic K-ATPase activity in the absence of inhibitor by Student’s t-test.

Fig. 2 — K-ATPase activity in the colonic membrane vesicles of mice. (A) Colonic K-ATPase activity was determined in the colonic membrane vesicles of HKα₂ wild type (WT), HKα₂ knockout (α₂ KO), and HKβ knockout (β KO) mice. Values (in micromole ATP hydrolyzed per mg membrane protein per hour) are means ± se from the indicated number of animals (n). Comparisons between groups were performed by Student’s t-test. NS stands for no statistically significant difference between groups. (B) K-dependent ATPase activity was measured in the colonic membrane vesicles extracted from wild type mice. Maximal stimulation of K-ATPase activity was reached at K⁺ concentration of 200 μM. Inset, Lineweaver-Burk plot of 1/V versus 1/[S] indicates apparent K_m of 40 μM for the colonic K-ATPase activity. (C) Colonic K-ATPase activity was measured in the absence of inhibitor or in the presence of 2 mM ouabain, or 100 μM Sch28080, or 100 μM orthovanadate. Values are means ± se from the indicated number of animals (n). * indicates P<0.001, statistically different from the colonic K-ATPase activity in the absence of inhibitor by Student’s t-test.