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. Author manuscript; available in PMC: 2016 Mar 5.
Published in final edited form as: Mol Cell Endocrinol. 2015 Jan 14;403:30–38. doi: 10.1016/j.mce.2015.01.015

Fig. 5.

Fig. 5

Coexpression of Twist1 and NICD1 in the developing limb. Immunochemistry of the E11.5 mouse limb bud. (A) Endogenous NICD1 expression in normal limb bud. (B) Positive labeling for NICD1 is pointed out by black arrow in the distal anterior peripheral mesenchyme. (C) A large population of cells in posterior limb bud was stained by NICD1. (D) Endogenous Twist1 expression in normal limb bud. (E) Positive labeling for Twist1 is restricted to the region of distal mesenchyme underlying the AER. (F) Cells in posterior limb bud were stained positive for Twist1. Scale bars, 50 µm.