. Author manuscript; available in PMC: 2016 Feb 12.

Published in final edited form as: J Med Chem. 2015 Jan 23;58(3):1307–1319. doi: 10.1021/jm5015742

Table 2.

Enzymatic activity of recombinant TbNTR and TcNTR toward Bnz and representative 3-nitrotriazoles from Table 1.

compound	Specific activity values (nmol NADH oxidized per min per mg NTR)

	TbNTR	TcNTR
Bnz	1540 ± 67	680 ± 8
2	3172 ± 11	888 ± 24
14	3456 ± 6	1067 ± 16
18	3063 ± 43	1178 ± 10
19	2681 ± 21	1160 ± 19

A precipitation issue occurred with compound 2 in the assay buffer used. Enzyme assays were carried out in triplicate and repeated twice.