|
| |||
| Component | Final concentration | Stock | Amount |
|
| |||
| Tris–HCl, pH 8.0 | 100 mM | 1 M | 25 ml |
|
| |||
| NaCl | 500 mM | 5 M | 25 ml |
|
| |||
| Add water to 250 ml | |||
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