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. Author manuscript; available in PMC: 2015 Feb 23.
Published in final edited form as: Methods Enzymol. 2014;541:27–34. doi: 10.1016/B978-0-12-420119-4.00003-3
Component Final concentration Stock Amount
NaCl 137 mM 5 M 1.37 ml
KCl 2.7 mM 4 M 33.8 μl
NaH2PO4 10 mM 0.5 M 1 ml
K2HPO4 2 mM 0.5 M 0.2 ml
Add water to 50 ml